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checkpoint 2 for presentation
Description
Subject Chosen for this Presantation:
Monoclonal Antibody Production via Hybridomas
Objectives
- CLO1: Apply protocols for basic laboratory techniques to completion, troubleshooting, and analysis of an experiment.
- CLO2: Analyze the rationale for and application of standard laboratory methods in biotechnology applications.
- CLO3: Examine the applications of equipment and techniques central to biotechnology research.
- CLO4: Assess the use of statistical analysis and data interpretation to design experiments in biotechnology research.
- CLO5: Evaluate the influence of standard laboratory methodology on the development of advanced techniques in biotechnology research.
- CLO6: Describe recent advances in experimental design, analytical methodology, and instrumentation that drive innovation in biotechnology research.
Introduction
This assignment aims to highlight the relationship between the development of technologies used in the biotechnology industry and their core experimental techniques. The following menu will provide the guidelines and examples to complete this signature assessment for the Emerging Applications in Biotechnology course.
Guidelines
Final Video Presentation
- Refer to the Experimental videos in the list below, which are discussed in the course modules.
- Select a single experimental technique from the videos to serve as the basis for your presentation.
- Find a company or research firm utilizing your selected experimental technique as the basis for a developed technology or technology in development. A good starting point is using the List of Biotech Companies or a search engine.
- Submit your selected technique and company or research firm for review. Send your chosen technique using the link to M10: Checkpoint #1: Technique selection – Final Presentation.
- Prepare the Experimental and Company Survey Slides describing the experimental technique, a primary research application, and the company’s implementation method using the rubric below.
- Submit your slides using the following link: M13: Checkpoint #2: Experimental Survey Slides Submission.
Use the following document to understand what is required for each one of the checkpoints.
Please use the following links for specific instructions to help you complete what is needed for these tasks. Each checkpoint will provide you with a detailed rubric and a link to upload your deliverables:
- Refer to the Experimental videos in the list (use left-side menu), which are discussed in the course modules.
- Select a single experimental technique from the videos to serve as the basis for your presentation.
- Find a company or research firm utilizing your selected experimental technique as the basis for a developed technology or technology in development.
- Submit your selected technique and company or research firm for review. Send your chosen technique using the link to M10: Checkpoint #1: Technique selection – Final Presentation.j
just watch this videos on youtoub
List of the Experimental Videos Available in this Course
- M03: TChIP-Seq: Cell-Type-Specific Epigenome Profiling
- M03: Substrate Generation for Endonucleases of CRISPR/Cas Systems
- M04: Generation of Transgenic Rats using a Lentiviral Vector Approach
- M04: Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
- M04: Targeted DNA Methylation Analysis by Next-generation Sequencing
- M04: Detection of Rare Mutations in CtDNA Using Next-Generation Sequencing
- M04: Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
- M04: Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
- M04: Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
- M04: Sequencing of mRNA from Whole Blood using Nanopore Sequencing
- M04: A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
- M05: Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
- M05: A Protocol for Computer-Based Protein Structure and Function Prediction
- M05: Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
- M05: Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
- M05: Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
- M06: Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
- M06: A Virtual Machine Platform for Non-Computer Professionals for Using Deep Learning to Classify Biological Sequences of Metagenomic Data
- M06: Novel Sequence Discovery by Subtractive Genomics
- M06: Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
- M06: Detection of Bacteria Using Fluorogenic DNAzymes
- M06: Zika Virus Specific Diagnostic Epitope Discovery
- M06: Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
- M09: Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
- M09: Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
- M10A: Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
- M10B: Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
- M10B: Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer
- M10B: Modeling Prostate Cancer in Genetically-engineered Mouse Models: A CRISPR/Cas9-mediated Localized Gene Editing Technique in Mouse Anterior Prostate Lobe Cells
- M10B: Modeling Prostate Cancer in Genetically-engineered Mouse Models: A CRISPR/Cas9-mediated Localized Gene Editing Technique in Mouse Anterior Prostate Lobe Cells
- M10B: Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes
- M10B: Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
- M10B: Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
- M10B: An Integrated Approach for Microprotein Identification and Sequence Analysis
- M10B: Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
- M10B: Culture of Bladder Cancer Organoids as Precision Medicine Tools
- M10B: Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine
- M10B: Large Animal Model for Evaluating the Efficacy of the Gene Therapy in Ischemic Heart
- M10B: CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy ApplicationsRefer to the Experimental videos in the list (use the left-side menu), which are discussed in the course modules.
Select a single experimental technique from the videos to serve as the basis for your presentation.
Find a company or research firm utilizing your selected experimental technique as the basis for a developed technology or technology in development. A good starting point is using the List of Biotech Companies or a search engine.
Submit your selected technique and company or research firm for review. Send your chosen technique using the link to M10: Checkpoint #1: Technique selection – Final Presentation.
Prepare the Experimental and Company Survey Slides describing the experimental technique, a primary research application, and the company’s implementation method using the rubric below.
Submit your slides using the following link: M13: Checkpoint #2: Experimental Survey Slides Submission.
Narrate and record a 10-minute video presentation.
Submit your Video Presentation using the following link to M15: Checkpoint #3: Final Video Presentation.
Experimental video: M09: Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Company or research firm utilizing technique: AstraZeneca and Sanofi
Product name: Beyfortus
Product description: Beyfortus is a new recombinant human immunoglobulin G1 kappa (IgG1κ) monoclonal antibody (mAb) approved to protect against respiratory syncytial virus (RSV) in infants and children up to 24 months
- M11B: Checkpoint #2: Experimental and Company Survey Slides – Final Presentation
- M12: Checkpoint #3: Final Video Presentation (Signature Assessment)
- Checkpoint #2: Experimental and Company Survey Slides
Due at the end of Module 11B
Each student must submit:
1. Experimental technique survey (6 slides)
Slide 1: Title slide
Slide 2: Name your selected technique and why you selected it.
- What interested you about the selected technique?
Slide 3: Describe the rational basis for your selected experimental technique (CLO2).
- What physical, chemical, or biological principles govern?
- How does your selected technique work?
Slide 4: Describe the basic procedure behind your selected experimental technique (CLO1).
- What steps and tools are needed to complete the experiment?
- What are the proper controls and basic assumptions?
Slide 5: Describe the process of validating your selected experimental technique (CLO4).
- What data is generated from your technique that allows you to determine whether it was successful?
- How do you determine success?
Slide 6: Summarize a primary research article that utilizes your selected technique (CLO3).
- What conclusions were the authors able to reach based on applying the selected technique?
- What additional knowledge was gained from using the technique in the article?
2. Company information summary (4 slides, plus bibliography)
Slide 7: Provide background on the company.
- When was the company founded?
- What is the focus of the company? (a certain disease, a specific treatment modality?)
- How large is the company?
Slide 8: Describe the product the company is developing.
- What is unique about the company’s approach?
- What are the potential applications?
- Who is the population utilizing this technology?
Slide 9: Describe how the product utilizes your selected experimental technique (CLO5).
- How is the discussed technique incorporated into or used by the highlighted product?
- What critical feature changes or modifications are made for the product (if any)?
Slide 10: Describe the results (up-to-date) of using the product (CLO6).
- What results have been generated using the product, and how do they indicate the effectiveness of the technology?
- How much more work is likely needed for the product to be commercialized?
Slide 11+: References slide (you may use multiple slides if needed)
- See the reference slide guidelines below
- Considerations for Slides
Each student must submit:
1. Experimental technique survey (6 slides)
Slide 1: Title slide
Slide 2: Name your selected technique and why you selected it.
- What interested you about the selected technique?
Slide 3: Describe the rational basis for your selected experimental technique (CLO2).
- What physical, chemical, or biological principles govern the technique?
- How does your selected technique work?
Slide 4: Describe the basic procedure behind your selected experimental technique (CLO1).
- What steps and tools are needed to complete the experiment?
- What are the proper controls and basic assumptions?
Slide 5: Describe the process of validating your selected experimental technique (CLO4).
- What data is generated from your technique that allows you to determine whether it was successful?
- How do you determine success?
Slide 6: Summarize a primary research article that utilizes your selected technique (CLO3).
- What conclusions were the authors able to reach based on applying the selected technique?
- What additional knowledge was gained from using the technique in the article?
2. Company information summary (4 slides, plus bibliography)
Slide 7: Provide background on the company.
- When was the company founded?
- What is the focus of the company? (a certain disease, a specific treatment modality?)
- How large is the company?
Slide 8: Describe the product the company is developing.
- What is unique about the company’s approach?
- What are the potential applications?
- Who is the population utilizing this technology?
Slide 9: Describe how the product utilizes your selected experimental technique (CLO5).
- How is the discussed technique incorporated into or used by the highlighted product?
- What critical feature changes or modifications are made for the product (if any)?
Slide 10: Describe the results (up-to-date) of using the product (CLO6).
- What results have been generated using the product, and how do they indicate the effectiveness of the technology?
- How much more work is likely needed for the product to be commercialized?
Slide 11+: References slide (you may use multiple slides if needed)
- See the reference slide guidelines (left-side menu)
As you develop your slides, you must consider the following:
- Who is your audience? Consider the area of knowledge and who the experts are, and then write to inform them.
- Defining the purpose. This is why, what, when, how, and who. Ensure each slide has a clear objective: to present data, illustrate a concept, or summarize findings. Avoid including extraneous information.
- Consider the structure of your slides. Limit each slide to one main idea or concept. Avoid cluttering with too much text or too many images.
- Format your slides consistently. Apply a uniform font style, size, and color scheme throughout your presentation. Each slide should have a descriptive title that conveys the main point. Label figures, graphs, and tables clearly.
- Use clear, relevant images to highlight the content. Select images that are informative and not cluttered. Ensure the image resolution and features are suitable for reading by your audience. Ensure visuals are clear, relevant, and appropriately labeled. Avoid overly complex graphs—simplify where possible and highlight key trends or data points.
- Emphasize key information. Use emphasis (e.g., bold text, color) to highlight important points or findings. Avoid excessive use of colors; use them purposefully to enhance understanding.
- Provide references. Cite sources for data and images used on each slide.
- Each slide should have a clear conclusion. Provide the bottom line for the information presented.
Rubrics are provided to guide the students through all the steps to complete this Final Presentation. Please refer to the checkpoint submission pages to see detailed rubrics for each one of the tasks.
Criteria for Checkpoint #2: Experimental and Company Survey
- Slide 1: The title slide includes all the necessary components.
- Slide 2: Name your selected technique and why you selected it.
- Slide 3: Describe the rational basis for your selected experimental technique (Aligns with CLO2).
- Slide 4: Describe the basic procedure behind your selected experimental technique (Aligns with CLO1).
- Slide 5: Describe the process of validating your selected experimental technique (Aligns with CLO4).
- Slide 6: Summarize a primary research article that utilizes your selected technique (Aligns with CLO3).
- Slide 7: Provide background on the company.
- Slide 8: Describe the product the company is developing.
- Slide 9: Describe how the product utilizes your selected experimental technique (Aligns with CLO5).
- Slide 10: Describe the results (up-to-date) of using the product (Aligns with CLO6).
- Slide 11+: References
- Formatting: Appropriate length, spelling, grammar, and readability
Quick Guide for Primary and Review Research Resources
APA Format
Consistent APA formatting should be used for any/all references, particularly for journal articles:
- Formatting for APA citations: Easybib GuidesLinks to an external site.
- How to cite journal articles in APA format: Easybib How to cite a journal APA format/Links to an external site.
Additional guidelines
Formatting Guidelines for the references slide(s):
- Divide the references into sections based on slide number:
- References should be listed in alphabetical order
- Use asterisks (*) to denote any references from which images were pulled (be sure that all pictures are cited with full references)
JHU Library Guides
Use the following link to JHU guidelines for citations and references.
- Refer to the Experimental videos in the list below, which are discussed in the course modules.
- Select a single experimental technique from the videos to serve as the basis for your presentation.
- Find a company or research firm utilizing your selected experimental technique as the basis for a developed technology or technology in development. A good starting point is using the List of Biotech Companies or a search engine.
- Submit your selected technique and company or research firm for review. Send your chosen technique using the link to M10: Checkpoint #1: Technique selection – Final Presentation.
- Prepare the Experimental and Company Survey Slides describing the experimental technique, a primary research application, and the company’s implementation method using the rubric below.
- Submit your slides using the following link: M13: Checkpoint #2: Experimental Survey Slides Submission.
Important :
- Refer to the Experimental videos in the list below, which are discussed in the course modules.
- Select a single experimental technique from the videos to serve as the basis for your presentation.
- Find a company or research firm utilizing your selected experimental technique as the basis for a developed technology or technology in development. A good starting point is using the List of Biotech Companies or a search engine.
- Submit your selected technique and company or research firm for review. Send your chosen technique using the link to M10: Checkpoint #1: Technique selection – Final Presentation.
- Prepare the Experimental and Company Survey Slides describing the experimental technique, a primary research application, and the company’s implementation method using the rubric below.
- Submit your slides using the following link: M13: Checkpoint #2: Experimental Survey Slides Submission.